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a-helix acid angle axial ratio Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn complex conformation constant contribution corresponding Cotton effects creatine kinase denatured determined dialyzate diffusible components dilute dipole electron density ellipticity enzyme equilibrium estimate exciton experimental factor Fasman frequency function gm/ml grams helical content helix hemocyanin hemoglobin intensity interaction kinase Kratky lysozyme macromolecules magnetic measurements membrane method Moffitt mole molecular weight molecule myoglobin negative negative band nondiffusible observed obtained optical activity ORD curve osmometers osmotic pressure paramagnetic parameter partial specific volume particles peptide Phys Pilz plot poly-L-proline polymer polypeptides positive procedure protein protein solution pulse radius of gyration random coil residues rotational strength rotatory sample Schellman Section shape solvent solvent medium spectrum sphere studies subunits techniques temperature theoretical scattering curves Tinoco tion transition tyrosine volume change wavelength zero