Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 56
... tion into a weighed amount of dialyzate . Since the densities in each set of components are known from the density measurements after equilibration , the volume dilution is known almost exactly by Volume dilution = g gi 2 i = 1 ( E ) ...
... tion into a weighed amount of dialyzate . Since the densities in each set of components are known from the density measurements after equilibration , the volume dilution is known almost exactly by Volume dilution = g gi 2 i = 1 ( E ) ...
Page 437
... tion bands which give rise to only weak optical activity . In these cases , since CD can only be measured at the same wavelength as the absorp- tion bands , the concentration of the molecule cannot be increased to im- prove the signal ...
... tion bands which give rise to only weak optical activity . In these cases , since CD can only be measured at the same wavelength as the absorp- tion bands , the concentration of the molecule cannot be increased to im- prove the signal ...
Page 460
... tion and CD bands we see that since the dipole strength is a measure of the total probability that a transition will occur , then the rotational strength is a measure of the total difference in probability that a transi- tion occurs in ...
... tion and CD bands we see that since the dipole strength is a measure of the total probability that a transition will occur , then the rotational strength is a measure of the total difference in probability that a transi- tion occurs in ...
Contents
F Density and Composition | 16 |
Direct Volume Change | 22 |
Density | 32 |
Copyright | |
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a-helix absorption acid Amer angle B-structure band Biochem Biochemistry Biol c₂ calculated CD spectra Chem chemical potential chromophore coefficient conformation constant Cotton effects cross-section denaturation determined dialyzate difference diffusible components dilute Donnan electron density enzyme equation equilibrated equilibrium exciton experimental function gm/ml grams helical helix hemocyanin hemoglobin intensity interaction Kratky Kupke Linderstrøm-Lang macromolecules magnetic measurements membrane method mixture molal molecular weight molecule monomer myoglobin negative nondiffusible components nonideality number of moles obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides positive potential procedure protein component protein concentration protein solution radius of gyration random coil residues rotational strength salt sample Scatchard Schellman Section shape small-angle scattering solvent solvent medium spectrum sphere structure subunits temperature Tinoco tion transition v₂ volume change wavelength zero