Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 50
Page 1
... DNA from a few kilobases (kb) to more than 10 megabases (Mb) long (Orbach et al., 1988), extending the size range of resolution for DNA molecules orders of magnitude beyond conventional agarose electrophoresis. The ability to separate ...
... DNA from a few kilobases (kb) to more than 10 megabases (Mb) long (Orbach et al., 1988), extending the size range of resolution for DNA molecules orders of magnitude beyond conventional agarose electrophoresis. The ability to separate ...
Page 2
... DNA in an agarose gel is placed in an electric field, the DNA molecules become elongated and oriented with the electric field as they move through the gel (Smith et al., 1989; Schwartz and Koval, 1989). The leading end represents the ...
... DNA in an agarose gel is placed in an electric field, the DNA molecules become elongated and oriented with the electric field as they move through the gel (Smith et al., 1989; Schwartz and Koval, 1989). The leading end represents the ...
Page 3
A Practical Guide Bruce Birren, Eric Lai. molecules will not resolve from all the DNA in the sample that is equal to or greater than its size. Therefore, the switch time is the single most important parameter in determining which molecules ...
A Practical Guide Bruce Birren, Eric Lai. molecules will not resolve from all the DNA in the sample that is equal to or greater than its size. Therefore, the switch time is the single most important parameter in determining which molecules ...
Page 4
... DNA molecules migrate in an order that does not reflect increasing size. Some molecules will migrate faster than molecules which are actually smaller, a phenomenon known as band inversion. In most PFGE band inversion occurs only in a ...
... DNA molecules migrate in an order that does not reflect increasing size. Some molecules will migrate faster than molecules which are actually smaller, a phenomenon known as band inversion. In most PFGE band inversion occurs only in a ...
Page 5
... DNA. The sizes of the largest molecules that could be resolved in pulsed-field gels using a constant switch interval are shown for DNAs of three different size ranges. Reproducing the resolution depicted requires use of the gel ...
... DNA. The sizes of the largest molecules that could be resolved in pulsed-field gels using a constant switch interval are shown for DNAs of three different size ranges. Reproducing the resolution depicted requires use of the gel ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes