Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 76
Page vi
... DNA Marker Systems 76 Ill. Materials 79 IV. Restrictive Fragment length Polymorphism Markers 80 V. Cleavable Amplified Polymorphic Sequences 87 VI. Random Amplified Polymorphic DNA 88 VII. Microsatellite Markers (Simple Sequence Repeats ...
... DNA Marker Systems 76 Ill. Materials 79 IV. Restrictive Fragment length Polymorphism Markers 80 V. Cleavable Amplified Polymorphic Sequences 87 VI. Random Amplified Polymorphic DNA 88 VII. Microsatellite Markers (Simple Sequence Repeats ...
Page 19
... DNA in agarose, e.g., for preparing material for cloning, requires a slightly different strategy. Because the ... sequence that has an equal proportion of A/T:G/C, the frequency of any restriction site is given by 4”, where n equals the ...
... DNA in agarose, e.g., for preparing material for cloning, requires a slightly different strategy. Because the ... sequence that has an equal proportion of A/T:G/C, the frequency of any restriction site is given by 4”, where n equals the ...
Page 20
... sequence Application (example) Enzymes with 4 and 5 base recognition sites Dp,n I GmóAVTC Pseudomonas aeruginosa PAO ... DNA must precede transfer. Two methods of fragmenting the DNA can be used: either depurination with acid or exposure ...
... sequence Application (example) Enzymes with 4 and 5 base recognition sites Dp,n I GmóAVTC Pseudomonas aeruginosa PAO ... DNA must precede transfer. Two methods of fragmenting the DNA can be used: either depurination with acid or exposure ...
Page 50
... DNA also present elsewhere in the genome, (ii) clones from other chromosomes containing similar sequence (i.e., clones from gene families), and (iii) clones misidentified due to the inherently difficult task of purifying chDNA bands ...
... DNA also present elsewhere in the genome, (ii) clones from other chromosomes containing similar sequence (i.e., clones from gene families), and (iii) clones misidentified due to the inherently difficult task of purifying chDNA bands ...
Page 51
... DNA cloning (Zolan et al., 1992). Chromosome “subtraction” libraries are a ... sequence-specific cleavage techniques. Chromosome fragmentation can be used ... DNA cloning. For fungal genomes (20–40 Mb), entire chromosomes or entire ...
... DNA cloning (Zolan et al., 1992). Chromosome “subtraction” libraries are a ... sequence-specific cleavage techniques. Chromosome fragmentation can be used ... DNA cloning. For fungal genomes (20–40 Mb), entire chromosomes or entire ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes