Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 52
Page 2
... band resolution with minimum run time. Separation of the desired size range of DNA fragments depends on the time required for the molecules to fully reorient from one field direction to the other. The duration of each electric field ...
... band resolution with minimum run time. Separation of the desired size range of DNA fragments depends on the time required for the molecules to fully reorient from one field direction to the other. The duration of each electric field ...
Page 3
... bands of the lambda ladder in the left lane of each panel decreases as the switch time is increased. Therefore, obtaining optimal resolution on a PFG requires use of the minimal switch time necessary to effectively separate the ...
... bands of the lambda ladder in the left lane of each panel decreases as the switch time is increased. Therefore, obtaining optimal resolution on a PFG requires use of the minimal switch time necessary to effectively separate the ...
Page 4
... band inversion. In most PFGE band inversion occurs only in a small region of the gel, normally near the sample well, and therefore does not interfere with determination of sizes. In a FIGE gel, this reversal of the relationship between ...
... band inversion. In most PFGE band inversion occurs only in a small region of the gel, normally near the sample well, and therefore does not interfere with determination of sizes. In a FIGE gel, this reversal of the relationship between ...
Page 6
... band inversion. With switch time ramping, the DNA molecules migrate with a mobility that reflects the average of all the switch times used. Thus, the actual size range of molecules separated on a gel with ramped switch times can be ...
... band inversion. With switch time ramping, the DNA molecules migrate with a mobility that reflects the average of all the switch times used. Thus, the actual size range of molecules separated on a gel with ramped switch times can be ...
Page 9
... bands, bands less sharp. Sharper bands, slower migration. Slows DNA migration. Needs changing less frequently. Slows rate of DNA migration. migration rate of DNA and upper limit of size range separated. Increases resolution. Decreases ...
... bands, bands less sharp. Sharper bands, slower migration. Slows DNA migration. Needs changing less frequently. Slows rate of DNA migration. migration rate of DNA and upper limit of size range separated. Increases resolution. Decreases ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes