Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 38
Page v
... Blotting of Pulsed-Field Gels 19 VI. Troubleshooting Pulsed-Field Gels 22 References 23 2 Electrophoretic Karyotyping in Fungi 25 Ken Dewar, Louis Bernier, and Roger C. Levesque I. Introduction 25 ll. Choice of Sample Material 29 Ill ...
... Blotting of Pulsed-Field Gels 19 VI. Troubleshooting Pulsed-Field Gels 22 References 23 2 Electrophoretic Karyotyping in Fungi 25 Ken Dewar, Louis Bernier, and Roger C. Levesque I. Introduction 25 ll. Choice of Sample Material 29 Ill ...
Page 10
... gene or preparing a few blots of separated yeast chromosomes for mapping. FIGE has the advantage that, aside from the switching unit, it requires only standard components such as a gel box and power 10 Jennifer S. Lee et al.
... gene or preparing a few blots of separated yeast chromosomes for mapping. FIGE has the advantage that, aside from the switching unit, it requires only standard components such as a gel box and power 10 Jennifer S. Lee et al.
Page 17
... blotting, the proper amount is that which gives clear, well-resolved bands (see Chapter 2). In general, the “best-looking” PFG results from DNA samples that contain 20–50 ng per band, enough for ethidium bromide visualization but not ...
... blotting, the proper amount is that which gives clear, well-resolved bands (see Chapter 2). In general, the “best-looking” PFG results from DNA samples that contain 20–50 ng per band, enough for ethidium bromide visualization but not ...
Page 19
... Blotting. of. Pulsed-Field. Gels. Many applications of PFGE call for blotting and hybridization of the separated DNA. These molecules are too large to transfer from the gel effiTable 1.4 Useful Restriction Enzymes for large Fragment Cloning ...
... Blotting. of. Pulsed-Field. Gels. Many applications of PFGE call for blotting and hybridization of the separated DNA. These molecules are too large to transfer from the gel effiTable 1.4 Useful Restriction Enzymes for large Fragment Cloning ...
Page 50
... blots of PFGE-resolved chromosomes with clones from existing libraries can be used to build chromosome-specific sublibraries, or chDNAs can be excised and probed "We distinguish between specific and enriched libraries since it is only ...
... blots of PFGE-resolved chromosomes with clones from existing libraries can be used to build chromosome-specific sublibraries, or chDNAs can be excised and probed "We distinguish between specific and enriched libraries since it is only ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes