Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 60
Page v
... . Sample Preparation 30 IV. Constructing Electrophoretic Karyotypes 38 V. Applications of Electrophoretic Karyotyping 47 VI. Conclusion 52 References 53 3 Isolation and Analysis of High-Molecular-Weight DNA from Plants 61 Contents.
... . Sample Preparation 30 IV. Constructing Electrophoretic Karyotypes 38 V. Applications of Electrophoretic Karyotyping 47 VI. Conclusion 52 References 53 3 Isolation and Analysis of High-Molecular-Weight DNA from Plants 61 Contents.
Page vi
A Practical Guide Bruce Birren, Eric Lai. 3 Isolation and Analysis of High-Molecular-Weight DNA from Plants 61 Martin Ganal I. Introduction 6] ll. Materials 62 III. Procedures 63 IV. Pitfalls 66 References 72 4 Generating and Using DNA ...
A Practical Guide Bruce Birren, Eric Lai. 3 Isolation and Analysis of High-Molecular-Weight DNA from Plants 61 Martin Ganal I. Introduction 6] ll. Materials 62 III. Procedures 63 IV. Pitfalls 66 References 72 4 Generating and Using DNA ...
Page xiii
... isolate genes, with much of this progress being attributable to the concerted effort dubbed the Human Genome Project. However valuable the specific information gathered about the human genome proves to be, the greatest legacy of the ...
... isolate genes, with much of this progress being attributable to the concerted effort dubbed the Human Genome Project. However valuable the specific information gathered about the human genome proves to be, the greatest legacy of the ...
Page 14
... isolation. The methods, most often enzymatic, are specific to each organism and vary with differences in the nature of the cell wall. Detailed protocols for preparing high-molecular-weight DNA from a variety of organisms may be found ...
... isolation. The methods, most often enzymatic, are specific to each organism and vary with differences in the nature of the cell wall. Detailed protocols for preparing high-molecular-weight DNA from a variety of organisms may be found ...
Page 15
... isolation should be extensive enough to remove small DNA fragments as well as the reagents used for DNA preparation, which can severely inhibit enzyme activity. B. Procedures Additional procedures for preparation of high-molecular ...
... isolation should be extensive enough to remove small DNA fragments as well as the reagents used for DNA preparation, which can severely inhibit enzyme activity. B. Procedures Additional procedures for preparation of high-molecular ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes