Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 94
Page vi
... Markers 80 V. Cleavable Amplified Polymorphic Sequences 87 VI. Random Amplified Polymorphic DNA 88 VII. Microsatellite Markers (Simple Sequence Repeats, SSR) 96 VIII. Sequence-Based Polymorphism Assays 108 IX. Higher Multiplex Ratio ...
... Markers 80 V. Cleavable Amplified Polymorphic Sequences 87 VI. Random Amplified Polymorphic DNA 88 VII. Microsatellite Markers (Simple Sequence Repeats, SSR) 96 VIII. Sequence-Based Polymorphism Assays 108 IX. Higher Multiplex Ratio ...
Page 3
... markers, reducing the ability to determine accurate sizes. In all PFGs, regardless of the particular switch time used,. 45 Sec. 75 SeC. 105 Sec. -900 –577 –577 -440 -214 -214 48.5Figure 1.1 Switch time determines the resolution and size ...
... markers, reducing the ability to determine accurate sizes. In all PFGs, regardless of the particular switch time used,. 45 Sec. 75 SeC. 105 Sec. -900 –577 –577 -440 -214 -214 48.5Figure 1.1 Switch time determines the resolution and size ...
Page 4
... markers that extend beyond the range of the molecules being studied, (ii) using size markers that have unique sizes and are easily distinguished, such as yeast chromosomes, in addition to markers with regular spacing, such as “ladders ...
... markers that extend beyond the range of the molecules being studied, (ii) using size markers that have unique sizes and are easily distinguished, such as yeast chromosomes, in addition to markers with regular spacing, such as “ladders ...
Page 22
... marker migration to the published figure should provide confirmation that the size range of interest is being separated to the desired extent. However, otherwise reliable size markers may not produce clear bands when run under ...
... marker migration to the published figure should provide confirmation that the size range of interest is being separated to the desired extent. However, otherwise reliable size markers may not produce clear bands when run under ...
Page 28
... markers to their chromosomes, and create chromosome-enriched genomic or cDNA libraries is an asset to any study of genome structure. Since many fungi do not have mating systems which can be controlled within a laboratory environment ...
... markers to their chromosomes, and create chromosome-enriched genomic or cDNA libraries is an asset to any study of genome structure. Since many fungi do not have mating systems which can be controlled within a laboratory environment ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes