Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 86
Page xiii
... methods become essential when genetic maps and mapping resources are lacking. The genomes of some of the world's economically most important species of animals, plants, and pathogens are still very poorly understood, and limited funding ...
... methods become essential when genetic maps and mapping resources are lacking. The genomes of some of the world's economically most important species of animals, plants, and pathogens are still very poorly understood, and limited funding ...
Page xiv
... methods that underlie virtually all methods described in the rest of the book. The remaining chapters of the book consider different cloning strategies, concluding with an approach to genome mapping that integrates all the kinds of ...
... methods that underlie virtually all methods described in the rest of the book. The remaining chapters of the book consider different cloning strategies, concluding with an approach to genome mapping that integrates all the kinds of ...
Page 14
... methods, most often enzymatic, are specific to each organism and vary with differences in the nature of the cell wall. Detailed protocols for preparing high-molecular-weight DNA from a variety of organisms may be found within the other ...
... methods, most often enzymatic, are specific to each organism and vary with differences in the nature of the cell wall. Detailed protocols for preparing high-molecular-weight DNA from a variety of organisms may be found within the other ...
Page 15
... method that is effective for preparing chromosomes from Saccharomyces cerevisiae. (1) Inoculate 100 ml of YPD medium with 0.1 ml of a saturated culture and grow for approximately 16 hr to achieve cultures in late log or early stationary ...
... method that is effective for preparing chromosomes from Saccharomyces cerevisiae. (1) Inoculate 100 ml of YPD medium with 0.1 ml of a saturated culture and grow for approximately 16 hr to achieve cultures in late log or early stationary ...
Page 18
... methods, working with large DNA in solution instead of in solid agarose will lead to breakage of very long DNA ... method, used to produce very large restriction fragments, enzymes and buffers diffuse into the agarose plugs which ...
... methods, working with large DNA in solution instead of in solid agarose will lead to breakage of very long DNA ... method, used to produce very large restriction fragments, enzymes and buffers diffuse into the agarose plugs which ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes