Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
|
From inside the book
Results 1-5 of 33
Page v
... Plugs 18 V. Southern Blotting of Pulsed-Field Gels 19 VI. Troubleshooting Pulsed-Field Gels 22 References 23 2 Electrophoretic Karyotyping in Fungi 25 Ken Dewar, Louis Bernier, and Roger C. Levesque I. Introduction 25 ll. Choice of ...
... Plugs 18 V. Southern Blotting of Pulsed-Field Gels 19 VI. Troubleshooting Pulsed-Field Gels 22 References 23 2 Electrophoretic Karyotyping in Fungi 25 Ken Dewar, Louis Bernier, and Roger C. Levesque I. Introduction 25 ll. Choice of ...
Page 17
... plugs at fivefold the calculated density, at the calculated density, and at one-fifth the calculated density. This will ensure that one of the sample preparations will be within the desirable range. Note that for very large DNA ...
... plugs at fivefold the calculated density, at the calculated density, and at one-fifth the calculated density. This will ensure that one of the sample preparations will be within the desirable range. Note that for very large DNA ...
Page 18
... Plugs. A. General Principles In cases where DNA samples are to be separated as intact chromosomes, no treatment of the DNA beyond purification is required. In other cases, such as long-range restriction mapping or preparative uses for ...
... Plugs. A. General Principles In cases where DNA samples are to be separated as intact chromosomes, no treatment of the DNA beyond purification is required. In other cases, such as long-range restriction mapping or preparative uses for ...
Page 19
... plugs which remain solid throughout the procedure. Generating partial digests of DNA in agarose, e.g., for preparing material for cloning, requires a slightly different strategy. Because the diffusion of the enzymes into the plugs is ...
... plugs which remain solid throughout the procedure. Generating partial digests of DNA in agarose, e.g., for preparing material for cloning, requires a slightly different strategy. Because the diffusion of the enzymes into the plugs is ...
Page 32
... Plug Treatment Solutions a. Sorbitol-EDTA c. EDTA-3-Mercaptoethanol To prepare 1 liter (prepared Final concentration fresh. Final concentration To prepare 200 ml 1 M Sorbitol (pH 5.8) Mix 100 ml 2 M Sorbitol (pH 5.8), 50 mM EDTA (pH 8) ...
... Plug Treatment Solutions a. Sorbitol-EDTA c. EDTA-3-Mercaptoethanol To prepare 1 liter (prepared Final concentration fresh. Final concentration To prepare 200 ml 1 M Sorbitol (pH 5.8) Mix 100 ml 2 M Sorbitol (pH 5.8), 50 mM EDTA (pH 8) ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
Other editions - View all
Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes