Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
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Page vi
... Polymorphism Assays 108 IX. Higher Multiplex Ratio Assays: Amplified Fragment length Polymorphism and Interrepeat Amplification T TO X. Choosing Appropriate Technology 127 References 129 5 Genome Mapping of Protozoan Parasites by ...
... Polymorphism Assays 108 IX. Higher Multiplex Ratio Assays: Amplified Fragment length Polymorphism and Interrepeat Amplification T TO X. Choosing Appropriate Technology 127 References 129 5 Genome Mapping of Protozoan Parasites by ...
Page 48
... polymorphisms Ono and Ishino-Arao (1988) McCluskey and Mills (1990) Plummer and Howlett (1993) Russell and Mills (1993) Zolan et al. (1994) Dewar and Bernier (1995) Martin (1995a) Candida lusitaniae Tolypocladium inflatum C. tropicalis ...
... polymorphisms Ono and Ishino-Arao (1988) McCluskey and Mills (1990) Plummer and Howlett (1993) Russell and Mills (1993) Zolan et al. (1994) Dewar and Bernier (1995) Martin (1995a) Candida lusitaniae Tolypocladium inflatum C. tropicalis ...
Page 50
... polymorphisms in fungi, comparative electrophoretic karyotyping has been used widely in measuring genome plasticity: in measuring genome plasticity in wild-type populations, in investigating the effects of genome plasticity on phenotype ...
... polymorphisms in fungi, comparative electrophoretic karyotyping has been used widely in measuring genome plasticity: in measuring genome plasticity in wild-type populations, in investigating the effects of genome plasticity on phenotype ...
Page 54
... polymorphisms in Ophiostoma ulmi (sensulato). Curr Genet. 27, 541–549. Doebbeling, B. N., Lehmann, P. F., Hollis, R.J., Wu, L.-C., Widmer, A. F., Voss, A., and Pfaller, M. A. (1993). Comparison of pulsed-field gel electrophoresis with ...
... polymorphisms in Ophiostoma ulmi (sensulato). Curr Genet. 27, 541–549. Doebbeling, B. N., Lehmann, P. F., Hollis, R.J., Wu, L.-C., Widmer, A. F., Voss, A., and Pfaller, M. A. (1993). Comparison of pulsed-field gel electrophoresis with ...
Page 56
... polymorphisms in the genus Pythium. Mycologia 87, 333–353. Masel, A. M., Braithwaite, K, Irwin, J., and Manners, J. (1990). Highly variable molecular karyotypes in the plant pathogen Colletotrichum gloeosporioides. Curr Genet. 18, 81–86 ...
... polymorphisms in the genus Pythium. Mycologia 87, 333–353. Masel, A. M., Braithwaite, K, Irwin, J., and Manners, J. (1990). Highly variable molecular karyotypes in the plant pathogen Colletotrichum gloeosporioides. Curr Genet. 18, 81–86 ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes