Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 89
Page v
... Preparation and Analysis of Large DNA l Jennifer S. Lee, Bruce Birren, and Eric Lai I. Pulsed-Field Gel Electrophoresis (PFGE) l II. Materials 11 Ill. Preparation of Nonmammalian Chromosomes 13 IV. Enzymatic Reactions Using DNA-Agarose ...
... Preparation and Analysis of Large DNA l Jennifer S. Lee, Bruce Birren, and Eric Lai I. Pulsed-Field Gel Electrophoresis (PFGE) l II. Materials 11 Ill. Preparation of Nonmammalian Chromosomes 13 IV. Enzymatic Reactions Using DNA-Agarose ...
Page 1
... Preparation. and. Analysis. of. Large. DNA. Jennifer S. Lee, Bruce Birren, and Eric Lai. I. Pulsed-Field. Gel ... preparing and manipulating high-molecular-weight DNA. NONMAMMALIAN GENOMIC ANALYSIS: A PRACTICAL GUIDE l Copyright © 1996 by ...
... Preparation. and. Analysis. of. Large. DNA. Jennifer S. Lee, Bruce Birren, and Eric Lai. I. Pulsed-Field. Gel ... preparing and manipulating high-molecular-weight DNA. NONMAMMALIAN GENOMIC ANALYSIS: A PRACTICAL GUIDE l Copyright © 1996 by ...
Page 11
... the buffer or the gel, because ethidium bromide will slow down the migration of the DNA and alter the size range of fragments separated. 1. 1 × TBE Amount needed to prepare 1 liter Introduction to Pulsed-Field Gels |l II. Materials.
... the buffer or the gel, because ethidium bromide will slow down the migration of the DNA and alter the size range of fragments separated. 1. 1 × TBE Amount needed to prepare 1 liter Introduction to Pulsed-Field Gels |l II. Materials.
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... prepare 1 liter of a 10X Component Final concentration (mM) concentrated solution Tris 89 108 g Tris base Borate 89 55 g boric acid EDTA 25 9.3 g disodium EDTA-2 H2O H2O - Add to bring final volume to 1 liter The pH of this mixture will ...
... prepare 1 liter of a 10X Component Final concentration (mM) concentrated solution Tris 89 108 g Tris base Borate 89 55 g boric acid EDTA 25 9.3 g disodium EDTA-2 H2O H2O - Add to bring final volume to 1 liter The pH of this mixture will ...
Page 13
... prepare 1 liter of LB mix: 10 g Bacto-tryptone 5 g Bacto—yeast extract 10 g NaCl Add H2O to bring final volume to 1 ... preparation of DNA samples in solid agarose, highly purified and quality tested low-melting agarose (e.g., InCert ...
... prepare 1 liter of LB mix: 10 g Bacto-tryptone 5 g Bacto—yeast extract 10 g NaCl Add H2O to bring final volume to 1 ... preparation of DNA samples in solid agarose, highly purified and quality tested low-melting agarose (e.g., InCert ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes