Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 53
Page vii
... Chromosome-Specific DNA Clones from African Trypanosome. I. Introduction 223 ll. Materials 226 Ill. Protocols 229 IV. Summary 252 References 253 ll. Materials 32] Ill. Procedures 323 IV. Conclusions 343 References CONTENTS vii.
... Chromosome-Specific DNA Clones from African Trypanosome. I. Introduction 223 ll. Materials 226 Ill. Protocols 229 IV. Summary 252 References 253 ll. Materials 32] Ill. Procedures 323 IV. Conclusions 343 References CONTENTS vii.
Page xiii
... protocols for procedures exactly as they are carried out in the labs where they are successfully used. Since many of the users of this work will have limited prior exposure to molecular biology, let alone genomic analysis, the ...
... protocols for procedures exactly as they are carried out in the labs where they are successfully used. Since many of the users of this work will have limited prior exposure to molecular biology, let alone genomic analysis, the ...
Page 1
... protocols for preparing and manipulating high-molecular-weight DNA. NONMAMMALIAN GENOMIC ANALYSIS: A PRACTICAL GUIDE l Copyright © 1996 by Academic Press, Inc. All rights of reproduction in any form reserved. B. Size-Dependent ...
... protocols for preparing and manipulating high-molecular-weight DNA. NONMAMMALIAN GENOMIC ANALYSIS: A PRACTICAL GUIDE l Copyright © 1996 by Academic Press, Inc. All rights of reproduction in any form reserved. B. Size-Dependent ...
Page 14
... protocols for preparing high-molecular-weight DNA from a variety of organisms may be found within the other chapters of this book and elsewhere (Birren and Lai, 1993). While the exact protocol for isolation of large or chromosomal DNA ...
... protocols for preparing high-molecular-weight DNA from a variety of organisms may be found within the other chapters of this book and elsewhere (Birren and Lai, 1993). While the exact protocol for isolation of large or chromosomal DNA ...
Page 16
... protocol will yield sufficient material for hundreds of lanes in PFGs. (12) Prior to electrophoresis, wash samples in 50 mM EDTA, pH 8.0, or electrophoresis buffer using several changes of at least 5 ml. 2. Preparation of Chromosomal ...
... protocol will yield sufficient material for hundreds of lanes in PFGs. (12) Prior to electrophoresis, wash samples in 50 mM EDTA, pH 8.0, or electrophoresis buffer using several changes of at least 5 ml. 2. Preparation of Chromosomal ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes