Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 68
Page v
... Sample Material 29 Ill. Sample Preparation 30 IV. Constructing Electrophoretic Karyotypes 38 V. Applications of Electrophoretic Karyotyping 47 VI. Conclusion 52 References 53 3 Isolation and Analysis of High-Molecular-Weight DNA from ...
... Sample Material 29 Ill. Sample Preparation 30 IV. Constructing Electrophoretic Karyotypes 38 V. Applications of Electrophoretic Karyotyping 47 VI. Conclusion 52 References 53 3 Isolation and Analysis of High-Molecular-Weight DNA from ...
Page 3
... sample that is equal to or greater than its size. Therefore, the switch time is the single most important parameter in determining which molecules are resolved in a PFG. Each set of PFGE conditions will separate a specific size range of ...
... sample that is equal to or greater than its size. Therefore, the switch time is the single most important parameter in determining which molecules are resolved in a PFG. Each set of PFGE conditions will separate a specific size range of ...
Page 4
... sample well, and therefore does not interfere with determination of sizes. In a FIGE gel, this reversal of the relationship between mobility and size can occur at either the bottom or the top of the gel and can greatly reduce the amount ...
... sample well, and therefore does not interfere with determination of sizes. In a FIGE gel, this reversal of the relationship between mobility and size can occur at either the bottom or the top of the gel and can greatly reduce the amount ...
Page 13
... samples in solid agarose, highly purified and quality tested low-melting agarose (e.g., InCert agarose, FMC BioProducts) is of value only when the samples will be used subsequently for restriction digestion. For preparation of intact ...
... samples in solid agarose, highly purified and quality tested low-melting agarose (e.g., InCert agarose, FMC BioProducts) is of value only when the samples will be used subsequently for restriction digestion. For preparation of intact ...
Page 14
... samples are then treated with enzymes and detergents that will digest the cell wall, membranes, proteins, and other cellular debris, allowing them to diffuse out of the agarose, leaving only the nucleic acid. At its simplest, the ...
... samples are then treated with enzymes and detergents that will digest the cell wall, membranes, proteins, and other cellular debris, allowing them to diffuse out of the agarose, leaving only the nucleic acid. At its simplest, the ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes