Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 27
Page 2
... switch, the DNA reorients to align itself with the new field direction prior to beginning to migrate (Smith et al., 1990). Therefore, the DNA actually migrates in a series of brief steps of alternating direction, with the electric ...
... switch, the DNA reorients to align itself with the new field direction prior to beginning to migrate (Smith et al., 1990). Therefore, the DNA actually migrates in a series of brief steps of alternating direction, with the electric ...
Page 3
... switch time. As the switch time is lengthened, the size range of molecules that can be resolved increases. Figure 1.1 shows that, with all other gel parameters held constant, increasing the switch interval from 45 to 75 sec increases ...
... switch time. As the switch time is lengthened, the size range of molecules that can be resolved increases. Figure 1.1 shows that, with all other gel parameters held constant, increasing the switch interval from 45 to 75 sec increases ...
Page 4
... switch time used, there will be a portion of the gel in which DNA molecules migrate in an order that does not ... switch times. The decreased resolution accompanying longer switch times and the separation of greater size ranges of DNA ...
... switch time used, there will be a portion of the gel in which DNA molecules migrate in an order that does not ... switch times. The decreased resolution accompanying longer switch times and the separation of greater size ranges of DNA ...
Page 5
... switch time for separating different sized DNA. The sizes of the largest molecules that could be resolved in pulsed-field gels using a constant switch interval are shown for DNAs of three different size ranges. Reproducing the ...
... switch time for separating different sized DNA. The sizes of the largest molecules that could be resolved in pulsed-field gels using a constant switch interval are shown for DNAs of three different size ranges. Reproducing the ...
Page 6
... switch time is progressively changed during the run, so that the fields alternate more frequently at the beginning of the run than at the end. This is termed switch time ramping. PFGs that are run with a constant switch time over the ...
... switch time is progressively changed during the run, so that the fields alternate more frequently at the beginning of the run than at the end. This is termed switch time ramping. PFGs that are run with a constant switch time over the ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes