Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
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Page vii
... Vector 199 IV. Construction of the Library 202 V. Sorting the Library 210 VI. Mapping Problems 217 VII. Summary 219 References 220 Construction of P1 Artificial Chromosome (PAC) Libraries from Lower Vertebrates 223 Chris T. Amemiya ...
... Vector 199 IV. Construction of the Library 202 V. Sorting the Library 210 VI. Mapping Problems 217 VII. Summary 219 References 220 Construction of P1 Artificial Chromosome (PAC) Libraries from Lower Vertebrates 223 Chris T. Amemiya ...
Page 8
... vector. Decreased mobility—longer run times; increases upper size limit of separation. Decreases upper size limit of separation; resolution increased. Using a narrower range of times for the ramp reduces the size range of fragments to ...
... vector. Decreased mobility—longer run times; increases upper size limit of separation. Decreases upper size limit of separation; resolution increased. Using a narrower range of times for the ramp reduces the size range of fragments to ...
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... vector. Proc. Nat Acad. Sci. U.S.A. 89, 8794-8797. Smith, C. L., Matsumoto, T., Niwa, O., Klco, S., Fan, J. B., Yanagida, M., and Cantor, C. R. (1987). An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel ...
... vector. Proc. Nat Acad. Sci. U.S.A. 89, 8794-8797. Smith, C. L., Matsumoto, T., Niwa, O., Klco, S., Fan, J. B., Yanagida, M., and Cantor, C. R. (1987). An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel ...
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... vectors. Science 236, 806-812. Burmester, A., and Wöstemeyer, J. (1994). Variability in genome organization of the zygomycete Parasitella parasitica. Curr Genet. 26, 456–460. Butler, D. K., and Metzenberg, R. L. (1990). Expansion and ...
... vectors. Science 236, 806-812. Burmester, A., and Wöstemeyer, J. (1994). Variability in genome organization of the zygomycete Parasitella parasitica. Curr Genet. 26, 456–460. Butler, D. K., and Metzenberg, R. L. (1990). Expansion and ...
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... vector for the propagation of large human DNA fragments. Nat. Genet.6, 84–89. Iwaguchi, S-I., Homma, M., and Tanaka, K. (1990). Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans. J ...
... vector for the propagation of large human DNA fragments. Nat. Genet.6, 84–89. Iwaguchi, S-I., Homma, M., and Tanaka, K. (1990). Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans. J ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes