Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
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Page vii
... Vector 199 IV. Construction of the Library 202 V. Sorting the Library 210 VI. Mapping Problems 217 VII. Summary 219 ... DNA Clones from African Trypanosome. I. Introduction 223 ll. Materials 226 Ill. Protocols 229 IV. Summary 252 ...
... Vector 199 IV. Construction of the Library 202 V. Sorting the Library 210 VI. Mapping Problems 217 VII. Summary 219 ... DNA Clones from African Trypanosome. I. Introduction 223 ll. Materials 226 Ill. Protocols 229 IV. Summary 252 ...
Page 8
... DNA PFGE, the buffer will not break down as rapidly as at the higher ... vector. Decreased mobility—longer run times; increases upper size limit of ... DNA >2Mb. continues Table 1.2 continued Function/ Parameters description Effect of ...
... DNA PFGE, the buffer will not break down as rapidly as at the higher ... vector. Decreased mobility—longer run times; increases upper size limit of ... DNA >2Mb. continues Table 1.2 continued Function/ Parameters description Effect of ...
Page 24
... DNA in Escherichia coli using an F-factor based vector. Proc. Nat Acad. Sci. U.S.A. 89, 8794-8797. Smith, C. L., Matsumoto, T., Niwa, O., Klco, S., Fan, J. B., Yanagida, M., and Cantor, C. R. (1987). An electrophoretic karyotype for ...
... DNA in Escherichia coli using an F-factor based vector. Proc. Nat Acad. Sci. U.S.A. 89, 8794-8797. Smith, C. L., Matsumoto, T., Niwa, O., Klco, S., Fan, J. B., Yanagida, M., and Cantor, C. R. (1987). An electrophoretic karyotype for ...
Page 55
... DNA: RecAassisted restriction endonuclease (RARE) cleavage. Science 254, 1494–1497. Ganal, M. W., Young, N. D., and ... vector for the propagation of large human DNA fragments. Nat. Genet.6, 84–89. Iwaguchi, S-I., Homma, M., and Tanaka ...
... DNA: RecAassisted restriction endonuclease (RARE) cleavage. Science 254, 1494–1497. Ganal, M. W., Young, N. D., and ... vector for the propagation of large human DNA fragments. Nat. Genet.6, 84–89. Iwaguchi, S-I., Homma, M., and Tanaka ...
Page 57
... vector for cloning high molecular weight DNA by the bacteriophage P1 system: Improved cloning efficacy. Proc. Natl. Acad. Sci., U.S.A. 89, 2056–2060. Plummer, K., and Howlett, B. J. (1993). Major chromosome length polymorphisms are ...
... vector for cloning high molecular weight DNA by the bacteriophage P1 system: Improved cloning efficacy. Proc. Natl. Acad. Sci., U.S.A. 89, 2056–2060. Plummer, K., and Howlett, B. J. (1993). Major chromosome length polymorphisms are ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes