Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
Results 1-5 of 59
Page 1
... yeast chromosomes can be separated in agarose gels by using two alternating electric fields of different orientation (Schwartz et al., 1982). This technology is now known as pulsed-field gel electrophoresis (PFGE). PFGE can resolve DNA ...
... yeast chromosomes can be separated in agarose gels by using two alternating electric fields of different orientation (Schwartz et al., 1982). This technology is now known as pulsed-field gel electrophoresis (PFGE). PFGE can resolve DNA ...
Page 3
... yeast chromosomes (right lane) were separated with switch intervals of 45, 75, or 105 sec. Each pulsedfield gel can separate a certain size range of DNA molecules, and lengthening the switch time increases this size range. However, as ...
... yeast chromosomes (right lane) were separated with switch intervals of 45, 75, or 105 sec. Each pulsedfield gel can separate a certain size range of DNA molecules, and lengthening the switch time increases this size range. However, as ...
Page 4
... yeast chromosomes, in addition to markers with regular spacing, such as “ladders,” and (iii) analysis of the fragments on gels of several different switch times. The decreased resolution accompanying longer switch times and the ...
... yeast chromosomes, in addition to markers with regular spacing, such as “ladders,” and (iii) analysis of the fragments on gels of several different switch times. The decreased resolution accompanying longer switch times and the ...
Page 10
... a particular gene or preparing a few blots of separated yeast chromosomes for mapping. FIGE has the advantage that, aside from the switching unit, it requires only standard components such as a gel box and power 10 Jennifer S. Lee et al.
... a particular gene or preparing a few blots of separated yeast chromosomes for mapping. FIGE has the advantage that, aside from the switching unit, it requires only standard components such as a gel box and power 10 Jennifer S. Lee et al.
Page 12
... yeast composed of yeast extract, peptone, and dextrose. To prepare 1 liter of YPD mix: 10 g Bacto—yeast extract 20 g Bacto-peptone Add H2O to 12 Jennifer S. Lee et al.
... yeast composed of yeast extract, peptone, and dextrose. To prepare 1 liter of YPD mix: 10 g Bacto—yeast extract 20 g Bacto-peptone Add H2O to 12 Jennifer S. Lee et al.
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes