Nonmammalian Genomic Analysis: A Practical GuideBruce Birren, Eric Lai Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
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From inside the book
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Page 34
... chromosomes for several reasons. (1) As shown by the number of fungi susceptible to Zymolyase treatment (Table 2.1) ... yeast artificial chromosomes. This procedure is based on spheroplasting before embedding. A similar protocol where ...
... chromosomes for several reasons. (1) As shown by the number of fungi susceptible to Zymolyase treatment (Table 2.1) ... yeast artificial chromosomes. This procedure is based on spheroplasting before embedding. A similar protocol where ...
Page 52
... artificial chromosomes (Shizuya et al., 1992; Ioannou et al., 1994), and yeast artificial chromosomes (Burke et al., 1987) will also be useful. The BAC and PAC systems have enormous potential in the mapping of fungal genomes: insert ...
... artificial chromosomes (Shizuya et al., 1992; Ioannou et al., 1994), and yeast artificial chromosomes (Burke et al., 1987) will also be useful. The BAC and PAC systems have enormous potential in the mapping of fungal genomes: insert ...
Page 61
... yeast artificial chromosomes suitable for chromosome walking (Guzman and Ecker, 1988, Ward and Jen, 1990; Grill and Sommerville, 1991; Martin et al., 1992, Kleine et al., 1993). NONMAMMALIAN GENOMIC ANALYSIS: A PRACTICAL GUli) E 6 ...
... yeast artificial chromosomes suitable for chromosome walking (Guzman and Ecker, 1988, Ward and Jen, 1990; Grill and Sommerville, 1991; Martin et al., 1992, Kleine et al., 1993). NONMAMMALIAN GENOMIC ANALYSIS: A PRACTICAL GUli) E 6 ...
Page 66
... yeast artificial chromosomes, bacteriophage P1, or bacterial artificial chromoSOrneS. IV. Pitfalls A. Protoplast Quality and Isolation The isolation of good quality protoplasts in a sufficiently high number is the most critical step in ...
... yeast artificial chromosomes, bacteriophage P1, or bacterial artificial chromoSOrneS. IV. Pitfalls A. Protoplast Quality and Isolation The isolation of good quality protoplasts in a sufficiently high number is the most critical step in ...
Page 73
... yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci. Mol. Gen. Genet, 223, 25–32. Martin, G. B., Brommonschenkel, S. H., Chungwonse, J., Frary, A., Ganal, M. W. ...
... yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci. Mol. Gen. Genet, 223, 25–32. Martin, G. B., Brommonschenkel, S. H., Chungwonse, J., Frary, A., Ganal, M. W. ...
Contents
1 | |
25 | |
61 | |
Chapter 4 Generating and Using DNA Markers in Plants | 75 |
Chapter 5 Genome Mapping of Protozoan Parasites by Linking Clones | 135 |
Chapter 6 Macrorestriction Mapping and Analysis of Bacterial Genomes | 165 |
Chapter 7 Cosmid Cloning with Small Genomes | 197 |
Chapter 8 Construction of PI Artificial Chromosome PAC Libraries from Lower Vertebrates | 223 |
Chapter 9 The Selection of ChromosomeSpecific DNA Clones from African Trypanosome Genomic Libraries | 257 |
Chapter 10 Analysis of the Dictyostelium discoideum Genome | 293 |
Chapter 11 Integrated Genome Mapping by Hybridization Techniques | 319 |
Index | 347 |
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Common terms and phrases
Acad AFLP agarose gel aliquots amplification bacterial bacteriophage bands blot buffer cells centrifuge cerevisiae chDNAs chromosomes coli colonies containing contigs cosmid Dictyostelium DNA fragments DNA molecules DNA sequence EDTA electrophoretic karyotype ethidium bromide filters Final concentration gel electrophoresis gene Genet genomic DNA hybridization identified Incubate insert isolation karyotype lane large DNA ligation linear linking clones markers method mg/ml molecular NaCl Natl Novozym Nucleic Acids Res nucleotide oligonucleotide PAC cloning partial digestion PFGE physical mapping plasmid plates plugs polymerase polymorphisms prepare primers probe procedure proteinase K protocol protoplasts pulse pulsed-field gel RAPD reaction repeat restriction digestion restriction endonuclease restriction enzyme restriction fragments restriction mapping resuspend RFLP room temperature samples selected separation Sfil solution Southern blot specific spheroplasts sterile strains switch techniques tion Tris-HCl trypanosome tube V/cm vector vector DNA yeast yeast artificial chromosomes