Transgenic Animals

Front Cover
CRC Press, May 22, 1997 - Nature - 592 pages
During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues.

Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics.

Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.
 

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Contents

PART IOVERVIEW
1
THE TECHNIQUES FOR GENE TRANSFER
7
Techniques and Problems in Producing Transgenic Pigs
15
The Generation of Transgenic Sheep by Pronuclear Microinjection
23
DNA Delivery by Cytoplasmic Injection
37
SECTION B IN WITRO MAMMAL EMBRYO MANIPULATION
45
In Vitro Generation of Bovine Embryos
51
The Use of Cryopreserved Fertilized Ova to Generate Transgenic Mammals
55
Insertional Mutagenesis in Transgenic Mice
361
EnhancerTrap Studies of the Drosophila Brain
365
An Overview
371
Model Organisms for the Analysis
387
The Preparation of Human Antibodies from Mice Harbouring Human
397
from Founder to an Established
403
The Use of Mouse Knockouts to Study Tumor Suppressor Genes
411
The Generation of New Immortalized Cell Lines through Transgenesis
421

The Microinjection of DNA into Early Chicken Embryo
69
The Use of Retroviral Vectors for Gene Transfer into Bird Embryo
83
Ballistic Transfection of Avian Primordial Germ Cells In Ovo
101
Transferring Foreign Genes into Zebrafish Eggs by Microinjection
119
SECTION E GENE TRANSFER INTO INVERTEBRATES
133
Gene Transfer Technology in Marine Invertebrates
151
SECTION F THE USE OF ES CELLS FOR GENE TRANSFER AND GENE TARGETING
157
Bovine Pluripotent Stem Cells for Transgenic Vectors
173
SECTION G THE DIRECT GENE TRANSFER INTO SOMATIC CELLS OF ANIMALS
189
Advances Toward Synthetic RetroVectoring
205
Chromosomal Insertion of Foreign DNA
219
Genetic Mosaicism in the Generation of Transgenic Mice
233
SECTION B THE VECTORS FORTRANSGENESIS
251
Transgene Rescue
267
Bacterial Artificial Chromosomes and Animal Transgenesis
283
Gene Transfer Using Microdissected Chromosome Fragments
299
Improvements in Transgene Expression by Gene Targeting
323
Reporter Genes and Detection of Mutational Activity in Mice
337
The Use of Transgenic Mammals for AIDS Studies
427
The Role of Mouse Models in the Development of New Therapies
435
Transgenic Animals in Atherosclerosis Research
445
The Generation and Use of Transgenic Animals for Xenotransplantation
455
THE PREPARATION OF RECOMBINANT PROTEINS FROM
461
Production of Complex Human Pharmaceuticals in the Milk of Transgenic
465
THE MODIFICATION OF MILK COMPOSITION
473
SECTION E THE PROTECTION OF ANIMALS AGAINST DISEASES
483
The Use of Antisense RNA and Ribozyme Sequences to Prevent
489
Antibody Encoding Transgenes Their Potential Use in Congenital
495
Potential Applications in Transgenic
501
Improved Wool Production from InsulinLike Growth Factor 1 Targeted
507
Recent Progress in Mammalian Genomics and its Application
519
THE NEW PROBLEMS GENERATED BY TRANSGENIC ANIMALS
525
The Relationalized Database of Transgenic Animals
533
The Patenting of Transgenic Animals
553
Ethical Problems of Animal Transgenesis
569
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