New Methods for Culturing Cells from Nervous TissuesPhilippe Poindron, Pascale Piguet, Dr. Eckart Förster "Presenting expert guidelines to prepare, establish and maintain neural cell and tissue cultures, this book will be a valuable tool for all scientists and technicians interested in basic and applied research in neurobiology, neurology and neuropharmacology."--BOOK JACKET. |
Contents
Protocol for the Primary Culture of Cortical Neurons | 12 |
A New Technique to Control the Architecture of Neuronal Networks | 23 |
Organotypic Cultures of the Rat Retina | 58 |
Cerebellar Slice Cultures | 74 |
Culture of Dissociated Hippocampal Neurons | 82 |
Improved Methods for Culturing Rat and Mouse Motor Neurons Their | 96 |
Characterization of LongTerm Cultured Cell Lines of Murine Microglia | 109 |
Author Index | 124 |
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Common terms and phrases
activity adhesion antibody axons BDNF Biol BioValley bipolar cells Brain Res calcium imaging cell bodies cell culture cell layer cell types cellular cerebellar slice cultures cerebellum CNTF coculture cortex cortical neurons culture medium dendritic density dentate gyrus differentiation dish dissection dry with N2 electrodes embryonic entorhinal excitatory explant cultures fibers fluorescence functional GABAergic glass coverslips granule cells HBSS hippocampal neurons immunocytochemistry incubated inhibitory interneurons Invitrogen kinase lithography MAMI-96 cells mannose receptor mask membrane mg/ml mice microglial cells microscope molecules morphology neocortex neural neurites neuron cultures neuronal networks neurons in culture Neurosci Neurosci Methods neurotrophic organotypic slice cultures pattern photoreceptors photoresist plasma plating Poindron poly-lysine postnatal preparation protein protocol Purkinje cells rat retina retina retinal cell Rinse serum serum-free Sigma solution Spin coating staining substrate survival synapses vitro vivo weeks in vitro Xaliproden µg/ml