Molecular cloning: a laboratory manual, Volume 2

Front Cover
CSHL Press, 2001 - Molecular cloning - 676 pages

From inside the book

Contents

Chapter
8-6
Chapter
8-17
INFORMATION PANELS
8-23
Analysis of Interacting Proteins with Surface Plasmon Resonance Spectroscopy 18
8-96
INFORMATION PANELS
8-107
INFORMATION PANELS
8-109
Chapter 9
8-120
Preparation of Radiolabeled DNA and RNA Probes 9 1
8-128
Exon Trapping and Amplification 11 80
11-80
INFORMATION PANELS
11-98
Chain Reaction
11-125
Chapter 1
12-1
Chapter 4
12-4
Chapter 12
12-12
Chapter 16
12-16
INTRODUCTION
12-32

Chapter 15
9-15
Chapter 18
9-18
INTRODUCTION
9-25
PROTOCOLS
9-33
INFORMATION PANELS
9-76
1
10-1
Chapter 10
10-10
Volume 1
10-11
Volume 2
10-16
INFORMATION PANELS
10-43
Chapter 3
11-3
Chapter 5
11-5
Chapter 11
11-7
Chapter 8
11-8
PROTOCOLS
11-13
INFORMATION PANELS
12-94
1
12-115
Chapter 13
13-13
INFORMATION PANELS
13-63
Purification of Closed Circular DNA by Equilibrium Centrifugation in CsClEthidium 1 69
13-69
INFORMATION PANELS
14-1
Chapter 2
14-2
Chapter 14
14-3
INFORMATION PANELS
14-9
INFORMATION PANELS
7
1
1-1
Chapter 7
1-7
Eukaryotic Cells
1-17
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Popular passages

Page 9-91 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 14-37 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 9-88 - Eldeiry, WS, Tokino, T., Velculescu. VE, Levy, DB, Parsons. R., Trent, JM, Lin, D., Mercer, WE, Kinzler, KW, and Vogelstein, B.
Page 9-92 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 9-87 - Campbell, AP, Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, ED, and Siebert, PD (1996).
Page 8-120 - Gilliland. G., Perrin. S., Blanchard. K., and Bunn, HF (1990) Analysis of cytokine mRNA and DNA detection and quantitation by competitive polymerase chain reaction.
Page 8-120 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 9-87 - DV, Angerer, LM, and Angerer, RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101, 485-502.
Page 12-103 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 9-88 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

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