Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

Front Cover
John Wiley & Sons, Dec 23, 2015 - Science - 736 pages

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.

This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
 

Contents

1 Introduction
1
2 Biology of Cultured Cells
19
3 Laboratory Design and Layout
37
4 Equipment and Materials
51
5 Aseptic Technique
73
6 Safety Bioethics and Validation
89
7 Culture Vessels and Substrates
111
8 Defined Media and Supplements
125
19 Differentiation
383
20 ThreeDimensional Culture
401
21 Scaleup and Automation
427
22 Senescence Immortalization and Transformation
455
23 Quantitation
483
24 Cytotoxicity
513
25 Culture of Specific Cell Types
531
26 Stem Cells
571

9 SerumFree Media
149
10 Preparation and Sterilization
173
11 Primary Culture
207
12 Subculture and Cell Lines
235
13 Authentication and Validation
259
14 Microbial Contamination
289
15 Cryopreservation and Banking
307
16 Cloning and Selection
327
17 Cell Sorting
347
18 Cell Line Characterization
359
27 Training Programs
591
28 Problem Solving
627
29 In Conclusion
651
Calculations and Preparation of Reagents
653
Glossary
663
Index
671
Links
685
Supplemental Images
1
EULA
33
Copyright

Other editions - View all

Common terms and phrases

About the author (2015)

R. IAN FRESHNEY, PhD, is an honorary Senior Research Fellow at the Instituteof Cancer Sciences at the University of Glasgow in Glasgow, UK. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

Bibliographic information