Inflammatory Bowel Disease: Current Status and Future Approach : Proceedings of the International Symposium on Future Research Approaches to Inflammatory Bowel Disease, Mechanisms of Chronic Infection and Inflammation, Fort Lauderdale, Florida, USA, October 7-11, 1987Richard P. MacDermott |
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Page 56
... ml ) were pipetted into flat - bottom wells of microtiter plates into which 0.1 ml of a 20 ug / ml dilution of Con A or 0.1 ml of RPMI 1640 + 20 % heat - inactivated FCS had been pipetted in triplicate . The cultures were incubated at ...
... ml ) were pipetted into flat - bottom wells of microtiter plates into which 0.1 ml of a 20 ug / ml dilution of Con A or 0.1 ml of RPMI 1640 + 20 % heat - inactivated FCS had been pipetted in triplicate . The cultures were incubated at ...
Page 234
... ml ) were cultured with PHA ( 1 μg / ml ) and supplemented on sequential days with either 0.1 ml of RPMI 1640 medium ... ug / ml trypsin or a - chymotrypsin for 30 minutes at 37 ° C , and the reaction stopped with 400 ug / ml soybean ...
... ml ) were cultured with PHA ( 1 μg / ml ) and supplemented on sequential days with either 0.1 ml of RPMI 1640 medium ... ug / ml trypsin or a - chymotrypsin for 30 minutes at 37 ° C , and the reaction stopped with 400 ug / ml soybean ...
Page 326
... ug / ml dose dependently inhibited PAF activity . The ID50 of prednisolone was 40 ug / ml . Salazopyrine also inhibited PAF synthesis and release in a dose dependent manner , the ID50 being 30 ug / ml . 5 - ASA dose dependently ...
... ug / ml dose dependently inhibited PAF activity . The ID50 of prednisolone was 40 ug / ml . Salazopyrine also inhibited PAF synthesis and release in a dose dependent manner , the ID50 being 30 ug / ml . 5 - ASA dose dependently ...
Contents
Defect in immunoregulatory intestinal epithelial cells in inflammatory | 9 |
Vasoactive intestinal peptide and the severity of colonic inflammation | 25 |
Substance P andor calcitonin generelated peptide are present | 43 |
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acid activity addition analysis animals antibodies antigen approach assay associated bacterial binding blood cell cultures changes chronic Clin clinical colon compared concentrations containing Crohn's disease culture cytotoxicity decreased demonstrated described detected determined Division effect epithelial cells examined experiments expression factor Figure function future Gastroenterology growth human immune Immunol increased incubated indicate induced infection inflammation inflammatory bowel disease inhibition intestinal isolated jejunum lamina propria levels lymph nodes lymphocytes MacDermott macrophages mean measured mechanisms mediators METHODS mice mucosal mycobacteria normal observed obtained occurred organisms patients positive prepared present previously production protein Publishers rats receptor REFERENCES release reported response role samples Science secretion sera serum showed shown significant similar specific specimens status stimulated strain studies suggest supported synthesis Table tested tissue ulcerative colitis vitro