PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 2
... Chapter 2 ) transformed the PCR into a simple and robust reaction which could now be automated by a thermal cycling device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could ...
... Chapter 2 ) transformed the PCR into a simple and robust reaction which could now be automated by a thermal cycling device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could ...
Page 4
... Chapter 4 . The ability to amplify specific sequences from crude DNA samples has important implications for research applications , ( e.g. , sperm lysates13 ; see Chapter 12 ) , for medical diagnostic applications ( e.g. , mouthwash1 or ...
... Chapter 4 . The ability to amplify specific sequences from crude DNA samples has important implications for research applications , ( e.g. , sperm lysates13 ; see Chapter 12 ) , for medical diagnostic applications ( e.g. , mouthwash1 or ...
Page 150
... Chapter 16. The use of this HLA typing system as well as of other PCR genetic markers in forensic analysis is the subject of Chapter 17 . The analysis of mutations in genes that effect cellular metabolism has contributed significantly ...
... Chapter 16. The use of this HLA typing system as well as of other PCR genetic markers in forensic analysis is the subject of Chapter 17 . The analysis of mutations in genes that effect cellular metabolism has contributed significantly ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube