PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 72
Principles and Applications for DNA Amplification Henry A. Erlich. DNA fragment enters the concentration of ... fragments differing by single base changes begin branching , and hence slowing down , at different positions in the gel ...
Principles and Applications for DNA Amplification Henry A. Erlich. DNA fragment enters the concentration of ... fragments differing by single base changes begin branching , and hence slowing down , at different positions in the gel ...
Page 78
... DNA fragments as large as 1,000 bp can be examined by DGGE , the method works best with DNA fragments 500 bp and smaller . Several different strategies can be used to choose the oligonucleotides for optimum results during both the PCR ...
... DNA fragments as large as 1,000 bp can be examined by DGGE , the method works best with DNA fragments 500 bp and smaller . Several different strategies can be used to choose the oligonucleotides for optimum results during both the PCR ...
Page 84
... DNA fragments on the high denaturant side of the gel barely enter the gel during electrophoresis because they melt extensively . At positions of the gel containing intermediate concentrations of denaturant , intermediate mobilities of the ...
... DNA fragments on the high denaturant side of the gel barely enter the gel during electrophoresis because they melt extensively . At positions of the gel containing intermediate concentrations of denaturant , intermediate mobilities of the ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube