PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 88
Page 179
... DNA sequencing . The success of the direct sequencing with 32P end - labeled DNA primers and a few negative experiences during attempts to obtain sequence ... DNA sequencer that employs end - labeled fluorescent DNA primers . 23,24 The ...
... DNA sequencing . The success of the direct sequencing with 32P end - labeled DNA primers and a few negative experiences during attempts to obtain sequence ... DNA sequencer that employs end - labeled fluorescent DNA primers . 23,24 The ...
Page 211
... DNA sequence of the amplified product using standard sequencing procedures 10-13 ( see Chapter 5 of this book ) . In cases where only a single sequence variant is present , e.g. , in mitochondrial DNA , direct sequencing of the PCR ...
... DNA sequence of the amplified product using standard sequencing procedures 10-13 ( see Chapter 5 of this book ) . In cases where only a single sequence variant is present , e.g. , in mitochondrial DNA , direct sequencing of the PCR ...
Page 245
Principles and Applications for DNA Amplification Henry A. Erlich. Index add - on sequence , 66 alu PCR from cloned genomic DNA , 116 amplification and DNA sequencing , 140 amplification plateau , 14 asymmetric primer ratio , 52 ...
Principles and Applications for DNA Amplification Henry A. Erlich. Index add - on sequence , 66 alu PCR from cloned genomic DNA , 116 amplification and DNA sequencing , 140 amplification plateau , 14 asymmetric primer ratio , 52 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube