PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 41
... DNA of a known sequence . The first example of this approach was the amplification of unknown cDNA sequences cloned into a λgt11 vector by using primers specific for the vector sequences flanking the insertion site.13 In general ...
... DNA of a known sequence . The first example of this approach was the amplification of unknown cDNA sequences cloned into a λgt11 vector by using primers specific for the vector sequences flanking the insertion site.13 In general ...
Page 62
... DNA . A few extra bases are added 5 ' to the restriction site to ensure that the efficiency of restriction enzyme ... sequences have been reported.2 This principle the introduction of DNA alterations via the PCR primers - is of great ...
... DNA . A few extra bases are added 5 ' to the restriction site to ensure that the efficiency of restriction enzyme ... sequences have been reported.2 This principle the introduction of DNA alterations via the PCR primers - is of great ...
Page 63
Principles and Applications for DNA Amplification Henry A. Erlich. Adding Useful Sequences 63 Similarly , fluorescent oligos can made 10 and used in PCR . Target - specific primers with different fluors can be used to distinguish ...
Principles and Applications for DNA Amplification Henry A. Erlich. Adding Useful Sequences 63 Similarly , fluorescent oligos can made 10 and used in PCR . Target - specific primers with different fluors can be used to distinguish ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube