PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 66
... Figures 2 and 3. Figure 2 shows a specific base substitution introduced as a mismatch between a PCR primer and the target sequence . This substitution is " moved " to the middle of a DNA fragment by the use of overlapping PCR products .
... Figures 2 and 3. Figure 2 shows a specific base substitution introduced as a mismatch between a PCR primer and the target sequence . This substitution is " moved " to the middle of a DNA fragment by the use of overlapping PCR products .
Page 75
... Figure 2B . A negative image of an ethidium - stained denaturing gradient gel of wild - type and mutant DNA fragments without and with a GC - clamp attached by PCR . The wild - type mouse B - globin promoter fragment without ( lane 1 ) ...
... Figure 2B . A negative image of an ethidium - stained denaturing gradient gel of wild - type and mutant DNA fragments without and with a GC - clamp attached by PCR . The wild - type mouse B - globin promoter fragment without ( lane 1 ) ...
Page 123
... Figure 3. Analysis of the sperm shown in Figure 2 . · genotype of the recombinant The PCR products shown in Figure 2 can be analyzed with two pairs of ASO's , one for each locus . and represent positive and negative hybridization . The ...
... Figure 3. Analysis of the sperm shown in Figure 2 . · genotype of the recombinant The PCR products shown in Figure 2 can be analyzed with two pairs of ASO's , one for each locus . and represent positive and negative hybridization . The ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube