PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 72
... GC - rich segment , which we call a GC - clamp , to a DNA fragment that melts in two domains.67 In the absence of the GC - clamp , only those single base changes that lie in the first melting domain of this DNA fragment were separated ...
... GC - rich segment , which we call a GC - clamp , to a DNA fragment that melts in two domains.67 In the absence of the GC - clamp , only those single base changes that lie in the first melting domain of this DNA fragment were separated ...
Page 75
... GC - clamp attached by PCR . The wild - type mouse B - globin promoter fragment without ( lane 1 ) and with ( lane 5 ) a 40 - bp GC - clamp . Three mutant promoter fragments without ( lanes 2-4 ) and with ( lanes 6-8 ) the same 40 - bp ...
... GC - clamp attached by PCR . The wild - type mouse B - globin promoter fragment without ( lane 1 ) and with ( lane 5 ) a 40 - bp GC - clamp . Three mutant promoter fragments without ( lanes 2-4 ) and with ( lanes 6-8 ) the same 40 - bp ...
Page 79
... GC - clamp . Melting predictions as well as our experience suggest that any random GC sequence will suffice . However , several suggestions may help prevent problems . Avoid repetitive stretches in the GC - clamp sequences that may lead ...
... GC - clamp . Melting predictions as well as our experience suggest that any random GC sequence will suffice . However , several suggestions may help prevent problems . Avoid repetitive stretches in the GC - clamp sequences that may lead ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube