PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 172
... ( HPRT ) . The large size of dystrophin and the predominance of DNA deletions that occur at this locus contrasts with the smaller HPRT gene that is frequently inactivated by point mutation . 8 rearrangements . Approximately 80 % of LN ...
... ( HPRT ) . The large size of dystrophin and the predominance of DNA deletions that occur at this locus contrasts with the smaller HPRT gene that is frequently inactivated by point mutation . 8 rearrangements . Approximately 80 % of LN ...
Page 172
... HPRT 1.6 Kb mRNA HPRT gene 26 | 25 | 24 | 23 || 22 21 13 12 11 11 21 22 ISSI I T XJ1.1 PERT 87 0.5 1.0 JBir p20 1.5 14 Kb mRNA DMD X Chromosome ( 150 Mb ) J66 3 ' DMD gene 2.0 ( Mb ) Figure 1. Organization of the genes defective in ...
... HPRT 1.6 Kb mRNA HPRT gene 26 | 25 | 24 | 23 || 22 21 13 12 11 11 21 22 ISSI I T XJ1.1 PERT 87 0.5 1.0 JBir p20 1.5 14 Kb mRNA DMD X Chromosome ( 150 Mb ) J66 3 ' DMD gene 2.0 ( Mb ) Figure 1. Organization of the genes defective in ...
Page 174
... HPRT peptide coding sequence and the presence of sufficient HPRT mRNA in most lymphoblastoid cell lines derived from LN patients to allow the PCR amplification of HPRT complementary DNA ( cDNA ) . The overall scheme is illustrated in ...
... HPRT peptide coding sequence and the presence of sufficient HPRT mRNA in most lymphoblastoid cell lines derived from LN patients to allow the PCR amplification of HPRT complementary DNA ( cDNA ) . The overall scheme is illustrated in ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube