PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 78
... PCR amplification and DGGE . In addition , three different approaches for determining the optimum denaturing gradient and the length of time of electrophoresis for each amplified DNA fragment can be used . OLIGONUCLEOTIDE DESIGN In ...
... PCR amplification and DGGE . In addition , three different approaches for determining the optimum denaturing gradient and the length of time of electrophoresis for each amplified DNA fragment can be used . OLIGONUCLEOTIDE DESIGN In ...
Page 80
... amplification , and the following suggestions are given . A. Spurious PCR amplification fragments : To use simple ethidium bromide staining to detect amplified DNA fragments on the denaturing gradient gels , it is necessary that the PCR ...
... amplification , and the following suggestions are given . A. Spurious PCR amplification fragments : To use simple ethidium bromide staining to detect amplified DNA fragments on the denaturing gradient gels , it is necessary that the PCR ...
Page 93
... amplified products which will hinder subsequent analysis . As little as 5 pmoles of primers have been use to give a very clean and efficient amplification . However , it is best to optimize the amount for each sequence you wish to amplify ...
... amplified products which will hinder subsequent analysis . As little as 5 pmoles of primers have been use to give a very clean and efficient amplification . However , it is best to optimize the amount for each sequence you wish to amplify ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube