PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 64
Principles and Applications for DNA Amplification Henry A. Erlich. PCR 2 target DNA PCR 1 w / primer mutagenesis A PCR 2 w / primer mutagenesis mix , remove primers & denature / renature 5 ' 3 ' & 5 ' 3 ' 3 ' extension PCR w / outside ...
Principles and Applications for DNA Amplification Henry A. Erlich. PCR 2 target DNA PCR 1 w / primer mutagenesis A PCR 2 w / primer mutagenesis mix , remove primers & denature / renature 5 ' 3 ' & 5 ' 3 ' 3 ' extension PCR w / outside ...
Page 100
... primers were then paired with appropriate T7 or SP6 polymerase sequence primers to carry out a PCR amplification using total library cDNA as template . The PCR product thus generated should represent a portion of the cDNA coding for the ...
... primers were then paired with appropriate T7 or SP6 polymerase sequence primers to carry out a PCR amplification using total library cDNA as template . The PCR product thus generated should represent a portion of the cDNA coding for the ...
Page 116
... primer , especially since the identification of this pair of amplifiable Alu repeats will provide sequences that can be amplified with other primers to help determine the parameters important in this reaction . Additional primers ...
... primer , especially since the identification of this pair of amplifiable Alu repeats will provide sequences that can be amplified with other primers to help determine the parameters important in this reaction . Additional primers ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube