PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 62
... PCR product fragments . Up to 45 base add - on sequences have been reported.2 This principle the introduction of DNA alterations via the PCR primers - is of great utility . Most simply , it can be used to specifically label one PCR product ...
... PCR product fragments . Up to 45 base add - on sequences have been reported.2 This principle the introduction of DNA alterations via the PCR primers - is of great utility . Most simply , it can be used to specifically label one PCR product ...
Page 63
... PCR . Target - specific primers with different fluors can be used to distinguish products made from different loci . " ADDING USEFUL SEQUENCES TO THE ENDS OF PCR PRODUCT As shown in Figure 1 , a restriction site can be added to the 5 ...
... PCR . Target - specific primers with different fluors can be used to distinguish products made from different loci . " ADDING USEFUL SEQUENCES TO THE ENDS OF PCR PRODUCT As shown in Figure 1 , a restriction site can be added to the 5 ...
Page 69
... PCR products . It's also probably a good idea to not use too many cycles of PCR past where the " plateau " in yield is reached . III . IV . Removal of excess primers from PCRs . In order to favor production of the combined PCR product ...
... PCR products . It's also probably a good idea to not use too many cycles of PCR past where the " plateau " in yield is reached . III . IV . Removal of excess primers from PCRs . In order to favor production of the combined PCR product ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube