PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 160
... Southern blot analysis using dystrophin cDNA probes . This procedure of multiplex PCR for deletion analysis provides a rapid screening test which should detect the mutation in 50 % of affected males . The remaining families will still ...
... Southern blot analysis using dystrophin cDNA probes . This procedure of multiplex PCR for deletion analysis provides a rapid screening test which should detect the mutation in 50 % of affected males . The remaining families will still ...
Page 162
... Southern blot ; 2 ) affected male fetus ( - ) ; 3 ) unaffected male fetus ( + ) ; 4 ) +/- female control , known +/- from Southern blot . Amplification may also be obtained by titration of the magnesium concentration in the PCR reaction ...
... Southern blot ; 2 ) affected male fetus ( - ) ; 3 ) unaffected male fetus ( + ) ; 4 ) +/- female control , known +/- from Southern blot . Amplification may also be obtained by titration of the magnesium concentration in the PCR reaction ...
Page 165
... Southern blotting in this diagnostic work will not be eliminated , but we expect that the use of Southern blots in 1989 will decline to about 30-40 % of its use in 1987. PCR techniques will probably replace about 90 % of Southern blot ...
... Southern blotting in this diagnostic work will not be eliminated , but we expect that the use of Southern blots in 1989 will decline to about 30-40 % of its use in 1987. PCR techniques will probably replace about 90 % of Southern blot ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube