PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 18
... DNA polymerase activity from T. aquaticus with a specific activity of 200,000 U / mg ( S. Stoffel , in preparation ) which migrates on SDS - PAGE slightly faster than Phosphorylase B ( 97.3 kDa ) and which has an ... Taq DNA Polymerase.
... DNA polymerase activity from T. aquaticus with a specific activity of 200,000 U / mg ( S. Stoffel , in preparation ) which migrates on SDS - PAGE slightly faster than Phosphorylase B ( 97.3 kDa ) and which has an ... Taq DNA Polymerase.
Page 19
... DNA when only half of the available dNTPs are incorporated into DNA . It is likely that very low dNTP concentrations may adversely affect the processivity of Taq DNA polymerase . Furthermore , the precise concentration of free and ...
... DNA when only half of the available dNTPs are incorporated into DNA . It is likely that very low dNTP concentrations may adversely affect the processivity of Taq DNA polymerase . Furthermore , the precise concentration of free and ...
Page 21
... DNA polymerase from T. aquaticus , DNA polymerase activities from Bacillus stearothermophilus , 14,15 several other ... Taq and other thermostable DNA polymerases , as well as the identification and characterization of replication ...
... DNA polymerase from T. aquaticus , DNA polymerase activities from Bacillus stearothermophilus , 14,15 several other ... Taq and other thermostable DNA polymerases , as well as the identification and characterization of replication ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube