PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 18
... Taq DNA Polymerase has a relatively high temperature optimum ( T ) for DNA synthesis . Depending on the nature of the DNA template , we have found an apparent Tt of 75-80 ° C with a K approaching 150 nucleotides / sec ... Taq DNA Polymerase.
... Taq DNA Polymerase has a relatively high temperature optimum ( T ) for DNA synthesis . Depending on the nature of the DNA template , we have found an apparent Tt of 75-80 ° C with a K approaching 150 nucleotides / sec ... Taq DNA Polymerase.
Page 19
... Taq DNA polymerase . Furthermore , the precise concentration of free and enzyme - bound magnesium may affect the processivity of Taq polymerase as has been inferred for calf thymus DNA polymerases a and 8.9 Purified 94 kDa Taq DNA ...
... Taq DNA polymerase . Furthermore , the precise concentration of free and enzyme - bound magnesium may affect the processivity of Taq polymerase as has been inferred for calf thymus DNA polymerases a and 8.9 Purified 94 kDa Taq DNA ...
Page 53
... polymerase , modified T7 DNA polymerase ( Sequenase ) , and reverse transcriptase are given at the end of the chapter ( see Protocol A ) . SEQUENCING OF PCR PRODUCTS WITH Taq POLYMERASE 8 Taq polymerase is an ideal enzyme for DNA ...
... polymerase , modified T7 DNA polymerase ( Sequenase ) , and reverse transcriptase are given at the end of the chapter ( see Protocol A ) . SEQUENCING OF PCR PRODUCTS WITH Taq POLYMERASE 8 Taq polymerase is an ideal enzyme for DNA ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube