PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 46
... agarose gel electrophoresis : 1. For fragments in the size range 80-1000 bp , either a 3 % NuSieve 1 % regular agarose or an acrylamide gel will give sufficient resolution . 2. Cut out a thin slice of the desired PCR fragment ...
... agarose gel electrophoresis : 1. For fragments in the size range 80-1000 bp , either a 3 % NuSieve 1 % regular agarose or an acrylamide gel will give sufficient resolution . 2. Cut out a thin slice of the desired PCR fragment ...
Page 155
... agarose , 1 % agarose mini - gel . 11,12 thalassemia , most cases of Duchenne muscular dystrophy , 8-10 and some cases of hemophilia A12 and retinoblastoma.13 In nearly all of these examples , because the sequences of critical DNA ...
... agarose , 1 % agarose mini - gel . 11,12 thalassemia , most cases of Duchenne muscular dystrophy , 8-10 and some cases of hemophilia A12 and retinoblastoma.13 In nearly all of these examples , because the sequences of critical DNA ...
Page 167
... agarose , 1 % agarose mini - gel . In one lane , load ~ 1-2 μg of ØX174 DNA digested with HaelII ( size marker ) with BPB dye . For each PCR sample , load 10 μl of product with 1 μl of marker dye . Electrophoresis at 100 volts until BPB ...
... agarose , 1 % agarose mini - gel . In one lane , load ~ 1-2 μg of ØX174 DNA digested with HaelII ( size marker ) with BPB dye . For each PCR sample , load 10 μl of product with 1 μl of marker dye . Electrophoresis at 100 volts until BPB ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube