PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 166
... aliquot of supernatant and dilute 1:10 with distilled H2O and then use 4 λ of diluted sample in 100 1 PCR reaction . CVS and cultured cells : · Wash villi with TE buffer x 2 . • To washed villi add 20-30 λ ( depending on amount of villi ) ...
... aliquot of supernatant and dilute 1:10 with distilled H2O and then use 4 λ of diluted sample in 100 1 PCR reaction . CVS and cultured cells : · Wash villi with TE buffer x 2 . • To washed villi add 20-30 λ ( depending on amount of villi ) ...
Page 188
... aliquots of the primer template mixture into four appropriately labeled tubes ( 1T , 1C , 1G , etc. ) . Do this step on the bench . 5. Add 2.0 μl of the appropriate dideoxyterminator / Sequenase mix ( 80 : 8 μM [ USB ] with 0.5 U / μl ...
... aliquots of the primer template mixture into four appropriately labeled tubes ( 1T , 1C , 1G , etc. ) . Do this step on the bench . 5. Add 2.0 μl of the appropriate dideoxyterminator / Sequenase mix ( 80 : 8 μM [ USB ] with 0.5 U / μl ...
Page 221
... aliquot of the pellet for microscopic examination . Suspend pellet in digestion buffer and treat with proteinase K as described above . Digest for no more than 2 hr . Spin in microcentrifuge 1 min to pellet sperm heads . Remove ...
... aliquot of the pellet for microscopic examination . Suspend pellet in digestion buffer and treat with proteinase K as described above . Digest for no more than 2 hr . Spin in microcentrifuge 1 min to pellet sperm heads . Remove ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube