PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 60
Page 47
... alleles differ by a single point mutation , direct sequencing using a PCR primer will display the heterozygote position . However , direct sequencing of allelic templates differing by several point mutations or short insertion ...
... alleles differ by a single point mutation , direct sequencing using a PCR primer will display the heterozygote position . However , direct sequencing of allelic templates differing by several point mutations or short insertion ...
Page 201
... alleles that are more highly associated with a particular disease . For example , the DRw6 serotype has a relative risk of 2.5 for the autoimmune dermatologic disease , Pemphigus vulgaris ( PV ) , whereas a particular DQẞ allele , DQB1 ...
... alleles that are more highly associated with a particular disease . For example , the DRw6 serotype has a relative risk of 2.5 for the autoimmune dermatologic disease , Pemphigus vulgaris ( PV ) , whereas a particular DQẞ allele , DQB1 ...
Page 211
... allelic variants must first be determined . Once this has been established , ASO probes can be designed to detect these alleles by differential hybridization . See Chapter 16 ( this book ) for a complete discussion of ASO detection ...
... allelic variants must first be determined . Once this has been established , ASO probes can be designed to detect these alleles by differential hybridization . See Chapter 16 ( this book ) for a complete discussion of ASO detection ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube