PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 41
... amplification of unknown cDNA sequences cloned into a λgt11 vector by using primers specific for the vector sequences flanking the insertion site.13 In general , flanking sequences for priming can be added by ligation " or by ...
... amplification of unknown cDNA sequences cloned into a λgt11 vector by using primers specific for the vector sequences flanking the insertion site.13 In general , flanking sequences for priming can be added by ligation " or by ...
Page 93
... amplified products which will hinder subsequent analysis . As little as 5 pmoles of primers have been use to give a very clean and efficient amplification . However , it is best to optimize the amount for each sequence you wish to amplify ...
... amplified products which will hinder subsequent analysis . As little as 5 pmoles of primers have been use to give a very clean and efficient amplification . However , it is best to optimize the amount for each sequence you wish to amplify ...
Page 214
... amplification of the 242 - bp DQa target sequence . Instead , primer pairs were chosen that amplified 166 - bp and ... amplification product yield but does not lead to artifactual typing results , suggesting that undamaged DNA serves as ...
... amplification of the 242 - bp DQa target sequence . Instead , primer pairs were chosen that amplified 166 - bp and ... amplification product yield but does not lead to artifactual typing results , suggesting that undamaged DNA serves as ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube