PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 40
... approach in which a labeled RNA probe is hybridized to the amplified fragment and a mismatch revealed by identification of the RNAse A cleavage product by gel electrophoresis . A chemical method of cleaving probe - target duplexes at ...
... approach in which a labeled RNA probe is hybridized to the amplified fragment and a mismatch revealed by identification of the RNAse A cleavage product by gel electrophoresis . A chemical method of cleaving probe - target duplexes at ...
Page 47
... approach # 1 will require some previous knowledge of the distribution of restriction sites in the different PCR products . DIRECT SEQUENCING OF HETEROZYGOUS INDIVIDUALS When two alleles differ by a single point mutation , direct ...
... approach # 1 will require some previous knowledge of the distribution of restriction sites in the different PCR products . DIRECT SEQUENCING OF HETEROZYGOUS INDIVIDUALS When two alleles differ by a single point mutation , direct ...
Page 130
... approach to determining the physical order of DNA polymorphisms which are so tightly linked ( less than 1 % recombination ) that they cannot be resolved by pedigree analysis . This may be especially significant in the case of random ...
... approach to determining the physical order of DNA polymorphisms which are so tightly linked ( less than 1 % recombination ) that they cannot be resolved by pedigree analysis . This may be especially significant in the case of random ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube