PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 19
... assay inhibits DNA synthesis by 50 % . While several investigators have observed that inclusion of 10 % DMSO facilitates certain PCR assays , it is not clear which parameters of PCR are affected . The presence of DMSO may affect the Tm ...
... assay inhibits DNA synthesis by 50 % . While several investigators have observed that inclusion of 10 % DMSO facilitates certain PCR assays , it is not clear which parameters of PCR are affected . The presence of DMSO may affect the Tm ...
Page 227
... assay , have been used to detect and identify ras mutations in several tumor types . The results of these studies ... assay . These mutations were not present in the surrounding normal tissue . With the ODN hybridization assay , 28 of ...
... assay , have been used to detect and identify ras mutations in several tumor types . The results of these studies ... assay . These mutations were not present in the surrounding normal tissue . With the ODN hybridization assay , 28 of ...
Page 239
... assay is critically linked to the setting of the background cutoff level . PCR - based assays , not unlike other ... assay by assay basis . There will be diagnostic targets such as the human immunodeficiency virus ( HIV ) that will ...
... assay is critically linked to the setting of the background cutoff level . PCR - based assays , not unlike other ... assay by assay basis . There will be diagnostic targets such as the human immunodeficiency virus ( HIV ) that will ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube