PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 72
... base changes begin branching , and hence slowing down , at different positions in the gel , resulting in the separation of the fragments at the end of the electrophoretic run . DGGE can be used to detect single base changes in all but ...
... base changes begin branching , and hence slowing down , at different positions in the gel , resulting in the separation of the fragments at the end of the electrophoretic run . DGGE can be used to detect single base changes in all but ...
Page 184
... base by direct DNA sequencing requires the signal from two bases at a single site to be reliably distinguished from noise that may occur from non - specific termination events in the dideoxynucleotide sequencing reactions . Examples of ...
... base by direct DNA sequencing requires the signal from two bases at a single site to be reliably distinguished from noise that may occur from non - specific termination events in the dideoxynucleotide sequencing reactions . Examples of ...
Page 186
... base differences in human DNA . In each case , a 942 - base fragment flanking exon 17 ( Figure 5B ) was PCR - amplified and analyzed as follows . A. Digestion with restriction endonuclease BstNI ; 5 μl of the reaction was digested with ...
... base differences in human DNA . In each case , a 942 - base fragment flanking exon 17 ( Figure 5B ) was PCR - amplified and analyzed as follows . A. Digestion with restriction endonuclease BstNI ; 5 μl of the reaction was digested with ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube