PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 33
... blood rather than purified mononuclear cells , inhibition of PCR occurs with the addition of as little as 1 μl blood to a 0.1 - ml reaction . There is a noticeable precipitate in the reaction tubes and purified DNA " spiked " into these ...
... blood rather than purified mononuclear cells , inhibition of PCR occurs with the addition of as little as 1 μl blood to a 0.1 - ml reaction . There is a noticeable precipitate in the reaction tubes and purified DNA " spiked " into these ...
Page 37
... blood cells are present , resuspend in 1 ml lysis buffer , transfer to a 1.5 - ml microcentrifuge tube and proceed as in step 2 of Protocol B above . The DNA concentration in the final resuspension depends on the number of cells present ...
... blood cells are present , resuspend in 1 ml lysis buffer , transfer to a 1.5 - ml microcentrifuge tube and proceed as in step 2 of Protocol B above . The DNA concentration in the final resuspension depends on the number of cells present ...
Page 232
... Blood 69 : 1237-1241 . Needleman , S.W. , Kraus , M.H. , Srivastava , S.K. , Levine , P.H. , and Aaronson , S.A. ( 1986 ) Blood 67 : 753-757 . Hirai , H. , Kobayashi , Y , Mano , H. , Hagiwara , K. , Maru , Y. , Omine , M. , Mizoguchi ...
... Blood 69 : 1237-1241 . Needleman , S.W. , Kraus , M.H. , Srivastava , S.K. , Levine , P.H. , and Aaronson , S.A. ( 1986 ) Blood 67 : 753-757 . Hirai , H. , Kobayashi , Y , Mano , H. , Hagiwara , K. , Maru , Y. , Omine , M. , Mizoguchi ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube