PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 123
... cell were positive indicating that DNA contamination was insignificant . No sample hybridized with both probes indicating that only a single cell was introduced into each tube and that DNA from lysed BA or BS cells present in the co ...
... cell were positive indicating that DNA contamination was insignificant . No sample hybridized with both probes indicating that only a single cell was introduced into each tube and that DNA from lysed BA or BS cells present in the co ...
Page 216
... cell DNA and sperm DNA were isolated from two partial vaginal swabs and from cell debris derived from vaginal swabs by a differential lysis procedure . In the first step , epithelial cells were preferentially lysed in a digestion buffer ...
... cell DNA and sperm DNA were isolated from two partial vaginal swabs and from cell debris derived from vaginal swabs by a differential lysis procedure . In the first step , epithelial cells were preferentially lysed in a digestion buffer ...
Page 230
... cell.23 The subsequent progression of MDS to AML is associated with a gradual increase in blast cells , which reflects the outgrowth of a new leukemic cell clone.24 Arbitrarily , patients with more than 30 % immature blast cells in the ...
... cell.23 The subsequent progression of MDS to AML is associated with a gradual increase in blast cells , which reflects the outgrowth of a new leukemic cell clone.24 Arbitrarily , patients with more than 30 % immature blast cells in the ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube