PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 32
Page 25
... changes and incubations . The first approach , typically robotic , has been used in a variety of formats , from ... change is dialed in . Third , control of the rate of temperature change in the samples is impossible during the transport ...
... changes and incubations . The first approach , typically robotic , has been used in a variety of formats , from ... change is dialed in . Third , control of the rate of temperature change in the samples is impossible during the transport ...
Page 72
... changes in the first two domains can be detected ; however , changes in the last domain are not generally detected due to the loss of sequence - dependent migration of the fragments upon complete strand dissociation . We were able to ...
... changes in the first two domains can be detected ; however , changes in the last domain are not generally detected due to the loss of sequence - dependent migration of the fragments upon complete strand dissociation . We were able to ...
Page 184
... change that is identified in an affected male with an X - linked recessive disease can be detected in other family ... changes based on competition between two oligonucleotide primers for DNA synthesis . Competitive oligonucleotide ...
... change that is identified in an affected male with an X - linked recessive disease can be detected in other family ... changes based on competition between two oligonucleotide primers for DNA synthesis . Competitive oligonucleotide ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
13 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube