PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 109
... chromosomes using a biotin - labeled , end - specific probe generated by inverse PCR . This probe contains approximately 1.3 kb of Drosophila DNA from the 3 ' end of a 120 - kb YAC that maps at the tip of chromosome 2R . Aside from ...
... chromosomes using a biotin - labeled , end - specific probe generated by inverse PCR . This probe contains approximately 1.3 kb of Drosophila DNA from the 3 ' end of a 120 - kb YAC that maps at the tip of chromosome 2R . Aside from ...
Page 110
... chromosomes of Drosophila melanogaster . The darkened region on the tip of chromosome 2R is the signal produced by hybridization to an inverse PCR product from a yeast artificial chromosome containing Drosophila genomic DNA . Most ...
... chromosomes of Drosophila melanogaster . The darkened region on the tip of chromosome 2R is the signal produced by hybridization to an inverse PCR product from a yeast artificial chromosome containing Drosophila genomic DNA . Most ...
Page 150
... chromosomal translocation which can be detected by PCR in a majority of cases because the breakpoints in most patients are clustered in a very small section of the chromosome . In the case of Philadelphia chromosome positive chronic ...
... chromosomal translocation which can be detected by PCR in a majority of cases because the breakpoints in most patients are clustered in a very small section of the chromosome . In the case of Philadelphia chromosome positive chronic ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube