PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 53
Page 109
... clones by inverse PCR for in situ hybridization , one can also determine the orientation of the insert DNA . In addition , many , if not most , YAC clones contain moderately or highly repetitive DNA sequences so they cannot be used ...
... clones by inverse PCR for in situ hybridization , one can also determine the orientation of the insert DNA . In addition , many , if not most , YAC clones contain moderately or highly repetitive DNA sequences so they cannot be used ...
Page 116
... clones overlap and contain only exons 7 , 8 and 9 , we predicted that the Alu repeats priming synthesis of this ... cloned DNAs from specific genomic regions in total human libraries and amplification from pulsed - field gel fragments ...
... clones overlap and contain only exons 7 , 8 and 9 , we predicted that the Alu repeats priming synthesis of this ... cloned DNAs from specific genomic regions in total human libraries and amplification from pulsed - field gel fragments ...
Page 117
... cloned DNAs . This has been particularly useful for the isolation of inserts in YAC clones , where the alternative methods are tedious and time consuming . Using this approach , we have generated fragments from about 75 % of YAC clones ...
... cloned DNAs . This has been particularly useful for the isolation of inserts in YAC clones , where the alternative methods are tedious and time consuming . Using this approach , we have generated fragments from about 75 % of YAC clones ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube