PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 70
Page 79
... containing more than a few adjacent G residues . As long as each PCR reaction is performed separately , it is satisfactory to use the same GC - clamp sequence for each set of primers . We have used six different GC - clamp sequences to ...
... containing more than a few adjacent G residues . As long as each PCR reaction is performed separately , it is satisfactory to use the same GC - clamp sequence for each set of primers . We have used six different GC - clamp sequences to ...
Page 129
... containing double sperm , and 2 ) the probability of amplifying an allele to a detectable level . The lowest frequency of recombination that can be measured with statistical reliability , therefore , is limited by the frequency of these ...
... containing double sperm , and 2 ) the probability of amplifying an allele to a detectable level . The lowest frequency of recombination that can be measured with statistical reliability , therefore , is limited by the frequency of these ...
Page 221
... containing 15 μl proteinase K solution . Incubate at least 1 h at 56 ° C . Remove blood stain substrate . Add 0.5 ml phenol / chloroform / isoamyl alcohol . Vortex . Spin in microcentrifuge 2 min . Transfer top ( aqueous ) phase to new ...
... containing 15 μl proteinase K solution . Incubate at least 1 h at 56 ° C . Remove blood stain substrate . Add 0.5 ml phenol / chloroform / isoamyl alcohol . Vortex . Spin in microcentrifuge 2 min . Transfer top ( aqueous ) phase to new ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
13 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube